nih3t3 mouse embryonic fibroblasts  (ATCC)

Journal: Cell Death Discovery

Article Title: Sibiriline, a novel dual inhibitor of necroptosis and ferroptosis, prevents RIPK1 kinase activity and (phospho)lipid peroxidation as a potential therapeutic strategy

doi: 10.1038/s41420-025-02852-8

Figure Lengend Snippet: A SH-SY5Y cells were treated with either 10 µM Erastin, 5 µM RSL3, 10 µM FIN56 or 25 µM FINO 2 , and increasing concentrations of Sib up to 50 µM or until reaching maximal cellular viability or 1 µM of ferrostatin-1 (Fer-1/F1) as a control. Cell viability was determined after 24 h of treatment, using the MTS assay. The bar graph represents the mean of two replicates. B SH-SY5Y cells were co-treated for 24 h with 5 µM RSL3 and increasing concentrations of Sib or Fer-1. Cell death was estimated by the lactate dehydrogenase (LDH) release assay. Results are plotted in % of LDH release measured in cells treated with RSL3 alone (left axis, colored blue). Cell viability was evaluated by MTS reduction assay. Results obtained (colored red) were plotted as % of maximal viability with DMSO-treated cells (right axis). Data are shown as the mean +/- SEM of three replicates. C NIH3T3 cells were treated with either 1 µM RSL3, 5 ng/ml TNFα and 20 µM z-VAD.fmk (TZ) or a combination of both treatment (TZ + RSL3) and 10 µM of Sib or Nec-1f, 30 µM Nec-1s or 1 µM of Fer-1. Cell viability was evaluated by MTS reduction assay after 16 h of treatment. Data are shown as the mean ± SEM of three replicates of two independent experiments. D NIH3T3 cells were treated with 5 ng/ml TNFα, 20 µM z-VAD.fmk and 1 µM RSL3 and increasing concentrations of Sib or Nec-1f. Cell viability was determined after 16 h of treatment using an MTS assay. Data are shown as the mean ± SEM of two replicates. EC 50 values were calculated using graphpad prism software. Statistical analysis was performed using two-way ANOVA and Tukey’s multiple comparisons test using graphpad prism software. * p < 0.05, ** p < 0.01, *** p < 0.001 and **** p < 0.0001 vs controls.

Article Snippet: Human neuroblastoma SH-SY5Y cell line, human fibrosarcoma HT1080 cell line, mouse fibroblast NIH3T3 cell line and mouse hippocampal HT-22 cell line were originally obtained from American Type Culture Collection (ATCC, Manassas, VA, USA).

Techniques: Control, MTS Assay, Lactate Dehydrogenase Assay, Software

Journal: PLOS One

Article Title: A novel sialylation pathway mediated by extracellular vesicles in aggressive prostate cancer

doi: 10.1371/journal.pone.0329014

Figure Lengend Snippet: (A) IB analysis of ST6GAL1 and actin in PC3 and TRAMP-C2 TCL using 1 μg/mL (right panel) or 2 μg/mL (left panel) of ST6GAL1 antibody; 40 µg of TCLs were used. (B) IB analysis of ST6GAL1, CNX and PDL1 in TRAMP-C2, RM1 and NIH3T3 TCLs; 85 µg of TCLs were used. A lane loaded with non relevant sample is included (Non relevant).

Article Snippet: Additionally, murine prostate adenocarcinoma cell lines TRAMP-C2 (ATCC, CRL-2731, RRID:CVCL_3615) and RM1 (provided by Dr.T.Thompson, Baylor College of Medicine, Houston, Texas, USA, RRID: CVCL_B459), and the mouse fibroblast cell line NIH3T3 (ATCC, CRL-1658, RRID: CVCL_0594) were used.

Techniques:

Journal: Materials Today Bio

Article Title: Antioxidant RuCo nanosheet attenuates capsule fibrosis in adhesive capsulitis of shoulder by p38 MAPK signaling pathway inhibition

doi: 10.1016/j.mtbio.2025.101979

Figure Lengend Snippet: ROS scavenging ability of RuCo-NSs. (a) ABTS assay demonstrated the ROS scavenging ability of RuCo-NSs. (b) H 2 O 2 scavenging ability and (c) O 2 •- scavenging ability of RuCo-NSs. (d) ESR assay demonstrated the •OH scavenging ability of RuCo-NSs. (e–f) Flow cytometry demonstrated the cytotoxicity of RuCo-NSs at various concentrations. (g–h) Intracellular ROS level was assessed by DCFH-DA. Bar = 100 μm. (i–j). GSH and MDA levels in NIH3T3s. Data represented mean ± SD. ∗p < 0.05.

Article Snippet: The mouse fibroblasts, NIH3T3s were obtained from ServiceBio.

Techniques: ABTS Assay, Flow Cytometry

Journal: Materials Today Bio

Article Title: Antioxidant RuCo nanosheet attenuates capsule fibrosis in adhesive capsulitis of shoulder by p38 MAPK signaling pathway inhibition

doi: 10.1016/j.mtbio.2025.101979

Figure Lengend Snippet: RuCo-NSs attenuated IL-1β-induced fibrogenesis in vitro . (a–b) EdU assay of NIH3T3s. Bar = 100 μm. (c–d) Wound healing assay of NIH3T3s. Bar = 100 μm. (e–f) Western blot showed the Col 1, VIM, and α-SMA expression in NIH3T3s. Data represented mean ± SD. ∗p < 0.05, ∗∗p < 0.01.

Article Snippet: The mouse fibroblasts, NIH3T3s were obtained from ServiceBio.

Techniques: In Vitro, EdU Assay, Wound Healing Assay, Western Blot, Expressing

Journal: Materials Today Bio

Article Title: Antioxidant RuCo nanosheet attenuates capsule fibrosis in adhesive capsulitis of shoulder by p38 MAPK signaling pathway inhibition

doi: 10.1016/j.mtbio.2025.101979

Figure Lengend Snippet: RuCo-NSs suppressed p38 MAPK signaling pathway both in vitro and in vivo . (a–b) Immunofluorescence analysis of p-p38 in NIH3T3s after different treatment. Bar = 100 μm. (c–d). Western blot showed the expression of p-p38 in NIH3T3s. (e–f) Immunofluorescence analysis of p-p38 in shoulder capsules. Bar = 100 μm. (g) Schematic depiction of the regulatory effect of RuCo-NSs on p38 MAPK signaling pathway. Data represented mean ± SD. ∗p < 0.05, ∗∗p < 0.01.

Article Snippet: The mouse fibroblasts, NIH3T3s were obtained from ServiceBio.

Techniques: In Vitro, In Vivo, Immunofluorescence, Western Blot, Expressing, Capsules